Background and objectives: The percutaneous route is an interesting and inventive\ninvestigation field of drug delivery. However, it is challenging for drug molecules to pass through\nthe skins surface, which is a characterized by its permeability barrier. The purpose of this study\nis to look at the effect of some penetration enhancers on in vivo permeation of insulin and insulin\nsensitizers (curcumin and rutin) through diabetes-induced mouse skin. Materials and Methods: Sting\ncrude extracts of Dendrocnide meyeniana, Urtica thunbergiana Sieb. and Zucc, and Alocasia odora (Lodd.)\nSpach were used as the penetration enhancers. Mouse skin irritation was tested by smearing the\nenhancers for the measurements at different time points and the cell viability of the HaCaT human skin\nkeratinocytes, which was determined by Trypan blue exclusion and MTT assays to evaluate human\nbiosafety for these extracts after the mouse skin permeation experiments. Results: All enhancers\ninduced a slight erythema without edema on the mouse skin that completely recovered after 6 h from\nthe enhancer smears as compared with normal mouse skin. Furthermore, no damaged cells were\nfound in the HaCaT keratinocytes under sting crude extract treatments. The blood sugar level in\nthe diabetic mice treated with the insulin or insulin sensitizers, decreased significantly (p < 0.05) in\nthe presence of enhancers................
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